so I thought we would have a week of experiments since dh needed to "clean his pipes" every day before our actual attempt, and of course a week is plenty of time to get it figured out right?! Yeah, that is if you don't ovulate a week early!!
So the night I ovulated we tried GDC again. The sperm in the centrifuge never seperated and where dead 60mins later.
We *think* the problem with the spinners where that the semen was too thick so next attempt will be adding canola oil to the semen before spinning.
Next attempt was the following afternoon....
seperated the sample to do both swim up and GDC
added canola oil to semen prior to spinning, they still didn't seperate and they all died. We think it's possible that spinning on high was too much stress, but still don't know why they didn't seperate.
the other sample went for swim up, the semen didn't seperate and they all died
The problem with the swim up was that the eggs were the same ph as
the semen and the incubator may have been too hot. DH's normal temp is
97.8 so keeping the incubator at 100.1 may have been too much
Third and final attempt was the same night and we only did swim up.
This time the eggs were 9 the sample was 8. Incubator temp was 98-99 and I put a cake pan full of boiling water to keep the humidity up.
There was definate swim up, but it appeared everything swam up, there was nothing in the bottom but egg white. The swimmers where still swimming!
So as a last ditch effort we took the top half of the semen and discarded and then added lime to the rest (which was also egg whites) till ph reached 5. It didn't appear any swimmers survived the lime but I instered anyway... an hour before did a lime douch and right before did a canola dip. I had intended to take it out (diva cup) in half and hour and check ph but fell asleep. 4hrs later woke up and checked diva cup ph and it was 6 left it out. 6 hours later vaginal ph was still 6.
I doubt this attempt was succesful but not sure where to go from here... and praying hard anyway!